Components of

Smallpox Vaccine

Components Page
Excipients Table
Allergens Table
Name ACAM2000
Manufacturer Sanofi Pasteur  
Viability Live attenuated
Indicated Ages 1 year and older  
Dose 0.0025 mL  
Schedule Single dose confirmed by lesion 6-8 days following vaccination. Booster every 3 years for high-risk.  
Route Percutaneous using bifurcated needle
Appearance Reconstituted vaccine should be a clear to slightly hazy, colorless to straw-colored liquid free from extraneous matter  
Concentration 1.0-5.0 x 108 plaque-forming units (PFU)/mL or 2.5-12.5 x 105 PFU/dose determined by plaque assay in Vero cells  
Preservative • Phenol 0.25%  
Adjuvant None
Excipients • HEPES (pH 6.5-7.5), 6-8 mM
• Human serum albumin USP, 2%
• Sodium chloride USP, 0.50.7%
• Mannitol USP, 5%
• Neomycin, trace amounts
• Polymyxin B, trace amounts
Allergens • Neomycin, trace amounts
• Polymyxin B, trace amounts
Media Vero (African green monkey kidney) cells  
Packaging Multiple-dose 3 mL clear glass vials containing lyophilized powder (freeze-dried vaccine).  
Storage 15 to -25°C (+5 to -13°F)
PI date Mar 2018  
  table last updated 4/8/19